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RT-PCR Protocols 2nd Edition

RT-PCR Protocols 2nd Edition


Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a

RT PCR Protocols Second Editionroutine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning.

In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

User friendly and up-to-date, RT-PCR Protocols 2nd Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.

The second edition of RT-PCR protocols and attempts to cover the most commonly used RT-PCR protocols, giving both beginners and experts a deeper understanding of the experimental set-up. There are often flow diagrams or pictures to aid understanding and the references are helpful, if you require additional information. A good investment for any lab interested in ensuring that their RT-PCR reactions are as productive and well-controlled as possible. Overall a useful and detailed text written in an informative and helpful style.

This is by far the best compendium of RT-PCR protocols I’ve seen thus far. Many such protocol books lack the detail necessary to repeat the experiments and leave many aspects to guesswork, which is time-consuming and expensive. The protocols delineated in this book are quite detailed, and serve as a good basis for most aspects of RT-PCR.

Download More: Understanding PCR A Practical Bench-Top Guide

This volume is not intended to be a hard-core technical manual that is accessible to a few Nobel Laureate molecular biologists. Rather, the goal is that it should act as a handy companion to anyone who wants to explore the marvels of gene expression. This includes students and their tutors, researchers, laboratory managers, and technologists from many diverse disciplines ranging from biochemistry to zoology and forensics to physiology.

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    • Book Name: RT-PCR Protocols 2nd Edition
    • File Type: PDF
    • Download: Link
    • Size: 9 MB
    • Password: PDFLibrary.Net

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